Introduction:Proliferative vitreoretinopathy (PVR), which is regulated by growth factors and cytokines, is the leading cause of failure in vitreoretinal surgery. In this study, we aimed to investigate the role of the human serum and vitreous inflammation-related factors in the development of proliferative vitreoretinopathy (PVR).

Methods:Blood and vitreous samples were obtained from patients undergoing pars plana vitrectomy. Inflammation-related factors were detected using an immunology multiplex assay on a Luminex^®xMAP^®platform. Patients with PVR and rhegmatogenous retinal detachment (RRD) were compared with macular hole (MH) or epiretinal membrane (ERM) patients without any other ocular or systemic disease.

Results:Thirty-six serum samples and 34 vitreous samples were obtained. Thirty-one different growth factors and cytokines were detected in serum samples. However, none of the circulating growth factors and cytokines were found to be different from the controls. Ten different growth factors and cytokines were measured in the vitreous samples. The concentration levels of PDGF-AA, TGF-α, VEGF, IL-6, IL-8, and TNFβ were found to have significantly increased in the vitreous of PVR patients.

Conclusion:Our study found that none of the circulating inflammation-related factors were changed in PVR or RRD patients, indicating the absence of a system inflammatory biomarkers to predict the development of proliferative vitreoretinopathy. As a supplement to previous research, the concentrations of PDGF-AA, TGF-α, VEGF, IL-6, IL-8, and TNFβ were significantly upregulated in the vitreous of PVR patients. These factors should be considered for preventing PVR.

本周为大家带来的文献为发表Adv Ther (IF: 3.4)的” Distinct Serum and Vitreous Inflammation-Related Factor Profiles in Patients with Proliferative Vitreoretinopathy”。本文使用了LabEx提供的Luminex检测服务。

 

增生性玻璃体视网膜病变（PVR）由生长因子和细胞因子调节，是玻璃体视网膜手术失败的主要原因。在该研究中，我们旨在探讨人类血清和玻璃体中的炎症相关因子在增生性玻璃体视网膜病变（PVR）发展中的作用。

 

血液和玻璃体样本取自接受睫状体平坦部玻璃体切除术的患者。使用Luminex®xMAP®平台上的免疫多重检测分析炎症相关因子。将患有增生性玻璃体视网膜病变（PVR）和孔源性视网膜脱离（RRD）的患者与无其他眼部或系统性疾病的黄斑裂孔（MH）或视网膜前膜（ERM）患者进行比较。

 

LabEx提供的Luminex检测服务,

 

根据制造商的说明，在 Luminex 平台上使用 MILLIPLEX MAP 人类细胞因子/凝血因子磁珠检测板检测所有样本。每孔使用 25 微升血清或玻璃体液进行分析。在血清和玻璃体样本中分别检测了 31 种和 10 种不同的分析物。

  

  

血清样本中的 31 种炎症因子概述

 

重要发现,

共获得36份血清样本和34份玻璃体样本。在血清样本中检测到31种不同的生长因子和细胞因子。然而，没有发现任何循环生长因子和细胞因子与对照组有显著差异。在玻璃体样本中测量了10种不同的生长因子和细胞因子。研究发现，PVR患者玻璃体中的PDGF-AA、TGF-α、VEGF、IL-6、IL-8和TNFβ的浓度水平显著增加。

 

研究表明，PVR或RRD患者的循环炎症相关因子没有变化，表明缺乏系统性炎症生物标志物来预测增生性玻璃体视网膜病变的发展。作为先前研究的补充，PVR患者玻璃体中的PDGF-AA、TGF-α、VEGF、IL-6、IL-8和TNFβ的浓度显著上调。这些因子应被考虑用于预防PVR。